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KMID : 1044720120020020098
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Journal of Lifestyle Medicine
2012 Volume.2 No. 2 p.98 ~ p.103
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CCN1 Induce Proliferation of Human Bone Marrow-Derived Stem Cells through PI3K/AKT Signaling Pathways
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Bae Sung-Hae
Kong Jee-Hyun
Shim Kwang-Yong
Lee Jong-In
Kim Hyun-Soo
Eom Young-Woo
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Abstract
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Background: The CCN1 protein is a secreted, cysteine-rich heparin-binding protein that associates with the cell surface and the extracellular matrix, and plays an important role in differentiation, migration, proliferation, and cell adhesion. However, little is known about the role of CCN1 in proliferation of mesenchymal stem cells.
Methods: To evaluate the changes of CCN1 expression during long-term culture of bone marrow-derived stem cells (BMSCs), cells were recovered at each passage and CCN1 mRNA expression was analyzed by RT-PCR and CCN1 protein levels were analyzed by western blot. BMSCs were treated with CCN1 proteins and a potent inhibitor of phosphoinositide 3-kinases (PI3K) to investigate the underlying mechanism for proliferation of stem cells.
Results: The secretion of CCN1 was dramatically decreased from passage 6 of BMSCs. CCN1 treatment induced proliferation of BMSCs and AKT activation but not ERK activation. LY294002, a potent inhibitor of PI3K suppressed AKT activation and decreased the proliferation of BMSCs.
Conclusions: Our results indicate that CCN1 can regulate the proliferation of mesenchymal stem cells through PI3K/AKT signaling pathways.
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KEYWORD
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CCN1, Bone marrow-derived stem cells, Proliferation, AKT activation
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